Serotype composition of Streptococcus pneumoniae in children with respiratory infections, optimization of molecular assessment methods

The paper presents optimized methods for PCR and sequence typing of Streptococcus pneumoniae. serotype composition pneumococci isolated from children under 5 years age with infections the upper respiratory tract was analyzed using methods. Between 2016 2021, there a decrease in frequency serotypes included pneumococcal 13-valent conjugate vaccine (PCV13) 94.1 to 25.8%, mainly due 6ABCD serogroup 19F serotype. coverage circulating PCV15 PCV20 vaccines 28.1% 41.6% respectively. During study period, number non-vaccine serogroups 11AD 15AF, as well that are not detected this capsular protocol, increased most significantly.